77热门这里精品无码_91精品国产制服啪啪无码_亚洲 欧美 国产 动漫 综合_三级片日本免费亚洲

159-2642-3062 027-65317797
服務(wù)熱線（工作日：9:00-17:30）

瀏覽量: 131

  • 產(chǎn)品名稱： 5-FAM Azide
  • 產(chǎn)品貨號： CSC306
  • 貨期： 現(xiàn)貨
  • 價格與訂購： 900
  • 數(shù)量：
    庫存： 10
  • 規(guī)格： 1 µmol
  • 產(chǎn)品信息
  • 如何訂購
    Introduction
    Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. The click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne. The azide and alkyne moieties can be used interchangeably; either one can be used to tag the molecule of interest, while the other is used for subsequent detection.
    The 5-FAM azide  is reactive with terminal alkyne via a copper-catalyzed click reaction that allows the subsequent visualization by fluorescence spectroscopy.
    Features
    Efficiency—the click reaction is complete in less than 1 hour;
    Specificity—the reaction between the label and detection tag is selective and specific;
    Stability—the reaction product contains an irreversible, covalent bond;
    Biologically inert—the components of the reaction do not undergo any side reactions.
    Figure 1. Click chemistry labeling
    Label
    FAM
    Ex/Em
    495/518 
    Detection Method
    Fluorescent 
    Solubility
    DMSO, DMF
    Molecular Weight
    532.51
    Product Size
    1 μmol 
    Storage Conditions
    -20 ℃, protect from light 
    Shipping Condition
    Room Temperature
    Applications
    Click chemistry labeling 
    Reference
    1.Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core.
    Dumont A, Malleron A, Awwad M, Dukan S, Vauzeilles B,Angew Chem Int Ed Engl (2012) 51:3143-3146
    2.Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry.
    Winz ML, Samanta A, Benzinger D, J?schke A,Nucleic Acids Res (2012) 40:e78-e78
    3.Mutant methionyl-tRNA synthetase from bacteria enables site-selective N-terminal labeling of proteins expressed in mammalian cells.
    Ngo JT, Schuman EM, Tirrell DA,Proc Natl Acad Sci U S A (2013) 110:4992-4997
    4.Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells.
    Hsu TL, Hanson SR, Kishikawa K, Wang SK, Sawa M, Wong CH Proc Natl Acad Sci U S A (2007) 104:2614-2619
    5.Metabolic labeling of sialic acids in living animals with alkynyl sugars.
    Chang PV, Chen X, Smyrniotis C, Xenakis A, Hu T, Bertozzi CR, Wu P Angew Chem Int Ed Engl (2009) 48:4030-4033
    Note
    For research use only .