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  • 產(chǎn)品名稱： Biotin DBCO
  • 產(chǎn)品貨號： CSC330
  • 貨期： 現(xiàn)貨
  • 價格與訂購： 1800
  • 數(shù)量：
    庫存： 10
  • 規(guī)格： 1 mg
  • 產(chǎn)品信息
  • 如何訂購
    Introduction
    Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. DBCO alkynes can be used to perform click reactions with azide-modified targets without the use of heavy metal catalysis. DBCO reactions are ideal for surface labeling of live cells and also minimize damage to fluorescent proteins like GFP or R-PE.
    The biotin DBCO is reactive with azide via a Strain-promoted Azide-Alkyne Click Chemistry reaction (SPAAC). Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin? biotin-binding protein.
    Features
    Efficiency—the click reaction is complete in less than 1 hour;
    Specificity—the reaction between the label and detection tag is selective and specific;
    Stability—the reaction product contains an irreversible, covalent bond;
    Biologically inert—the components of the reaction do not undergo any side reactions.
    Figure 1. Click chemistry labeling
    Specifications
    Label
    Biotin 
    Ex/Em
    – 
    Detection Method
    –
    Solubility
    DMSO, DMF 
    Molecular Weight
    747.95 
    Product Size
    1 mg
    Storage Conditions
    -20 ℃, protect from light 
    Shipping Condition
    Room Temperature 
    Applications
    Click chemistry labeling 
    Reference
    1.Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core.
    Dumont A, Malleron A, Awwad M, Dukan S, Vauzeilles B,Angew Chem Int Ed Engl (2012) 51:3143-3146
    2.Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry.
    Winz ML, Samanta A, Benzinger D, J?schke A,Nucleic Acids Res (2012) 40:e78-e78
    Note
    For research use only .