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  • 產(chǎn)品名稱： Anti Active Rap1 Mouse Monoclonal Antibody
  • 產(chǎn)品貨號： CST26912
  • 貨期： 現(xiàn)貨
  • 價格與訂購： 4800
  • 數(shù)量：
    庫存： 5
  • 規(guī)格： 100μg
  • 產(chǎn)品信息
  • 如何訂購
    Catalog Number
    CST26912
    Gene Symbol
    RAP1
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    Description
     Anti Active Rap1 Mouse Monoclonal Antibody
    Background
    Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway, Raf-1 and B-Raf. Rap1 regulates cell adhesion mechanisms through effector molecules such as the integrins and cadherins.
    Immunogen
    Recombinant full length protein of active Rap1.
    Applications
    IP, IHC
    Recommended Dilutions
    1 μg for ~2 mg total cellular proteins for IP 
    1:100 dilution for IHC, IF
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Isotype
    IgG
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Rap1 antibody recognizes active Rap1 of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Species Reactivity
    Anti active Rab7 antibody recognizes active Rab7 proteins of vertebrates.
    Immunoprecipitation/Western Blot

    Rap1 activation assay. Purified Rap1 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rap1 monoclonal antibody (Cat. No. 26912). Immunoblot was with an anti-Rap1 polyclonal antibody (Cat. No. 21104).                                                 
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.