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  • 產(chǎn)品名稱： Anti NFE2L2(D29G) Mouse Monoclonal Antibody
  • 產(chǎn)品貨號： CST26331
  • 貨期： 現(xiàn)貨
  • 價格與訂購： 4800
  • 數(shù)量：
    庫存： 5
  • 規(guī)格： 100μg
  • 產(chǎn)品信息
  • 如何訂購
    Catalog Number
    CST26331
    Gene Symbol
    NFE2L2, NRF2
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    Description
    Anti NFE2L2(D29G) Mouse Monoclonal Antibody
    Background
     NFE2L2 protein is encoded by NFE2L2 gene. It is a basic leucine zipper protein that regulates the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. Several drugs that stimulate the NFE2L2 pathway are being studied for treatment of diseases that are caused by oxidative stress.
    Immunogen
    A synthetic peptide from the internal region of NFE2L2 which includes the mutation of D29G, human origin.
    Applications
    ELISA, WB, IHC
    Recommended Dilutions
    ELISA: 1:1000-1:2000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity
    Recognizes D29G mutant, but not wild-type NFE2L2 of vertebrates.
    Immunoprecipitation/Western Blot

    Western blot analysis of recombinant NFE2L2(D29G) and wildtype proteins. Purified His-tagged NFE2L2(D29G) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti NFE2L2(D29G) monoclonal antibody (Cat. #26331).                                                       
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    Immunofluorescence: 

    Immunofluorescence of cells expressing NFE2L2 proteins with Anti NFE2L2(D29G) antibody. HEK293T cells were transfected with pCDNA3-GFP NFE2L2(WT) plasmid (left column) or pCDNA3-GFP NFE2L2(D29G) plasmid (right column), then fixed and stained with Anti NFE2L2(D29G) monoclonal antibody (Cat. #26331).
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.