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  • 產(chǎn)品名稱： Anti RAS(K117N) Mouse Monoclonal
  • 產(chǎn)品貨號： CST26468
  • 貨期： 現(xiàn)貨
  • 價格與訂購： 4800
  • 數(shù)量：
    庫存： 5
  • 規(guī)格： 100μg
  • 產(chǎn)品信息
  • 如何訂購
    Catalog Number
    CST26468
    Gene Symbol
    H-Ras; K-Ras; N-Ras
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    Description
    Anti RAS(K117N) Mouse Monoclonal Antibody
    Background
    The K117N mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras K117N mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
    Immunogen
    A synthetic peptide from the internal region of Ras which includes the mutation of K117N, human origin.
    Applications
    ELISA, WB, IHC
    Recommended Dilutions
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity
    Recognizes K117N mutant, but not wild-type RAS of vertebrates.
    Immunoprecipitation/Western Blot

    Western blot analysis of recombinant RAS(K117N) and wild-type proteins. Purified His-tagged RAS(K117N) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti RAS(K117N) monoclonal antibody (Cat. #26468).
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    Immunofluorescence: 

    Immunofluorescence of cells expressing KRas proteins with anti-KRAS(K117N) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRas (WT) plasmid (left column) or pCDNA3-GFP-KRAS(K117N) plasmid (right column), then fixed and stained with anti-KRAS(K117N) monoclonal antibody (Cat. #26468).
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.