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  • 產(chǎn)品名稱： Anti RET(C634Y) Mouse Monoclonal Antibody
  • 產(chǎn)品貨號(hào)： CST26146
  • 貨期： 現(xiàn)貨
  • 價(jià)格與訂購： 4800
  • 數(shù)量：
    庫存： 5
  • 規(guī)格： 100μg
  • 產(chǎn)品信息
  • 如何訂購
    Catalog Number
    CST26146
    Gene Symbol
    RET, PTC, CDHF12, CAHF16, HSCR1, MEN2A, MEN2B
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    Description
    Anti RET(C634Y) Mouse Monoclonal Antibody
    Background
    RET protein is encoded by the RET proto- oncogene. It is a receptor tyrosine kinase. RET loss of function mutations are associated with the development of Hirschsprung’s disease, while gain of function mutations are associated with development of various types of human cancer, including medullary thyroid carcinoma, multiple endocrine neoplasias type 2A and 2B, pheochromocytoma and parathyroid hyperplasia.
    Immunogen
    A synthetic peptide from the internal region of RET which includes the mutation of C634Y, human origin.
    Applications
     ELISA, WB, IHC
    Recommended Dilutions
    ELISA: 1:1000-1:2000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration
     0.5 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity
     Recognizes C634Y mutant, but not wild-type RET of vertebrates.
    Immunoprecipitation/Western Blot

    Western blot analysis of recombinant RET(C634Y) and wildtype proteins. Purified His-tagged RET(C634Y) protein (lane1) and corresponding wild-type protein (lane2) were blotted with Anti RET(C634Y) monoclonal antibody (Cat. #26146).    
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    Immunofluorescence: 

    Immunofluorescence of cells expressing RET proteins with Anti RET(C634Y) antibody. HEK293T cells were transfected with pCDNA3-GFP RET(WT) plasmid (left column) or pCDNA3-GFP RET(C634Y) plasmid (right column), then fixed and stained with Anti RET(C634Y) monoclonal antibody (Cat. #26146).
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.