瀏覽量: 135
- 產(chǎn)品名稱: ATG fect solution+ATGfeed medium
- 產(chǎn)品貨號(hào): CSO00018
- 貨期: 現(xiàn)貨
- 價(jià)格與訂購: 2800
- 數(shù)量:
- 規(guī)格:
- 產(chǎn)品信息
- 如何訂購
概述(Summary)
產(chǎn)品描述(Description)
ATG fect solution 是一種基于陽離子聚合物的優(yōu)異轉(zhuǎn)染試劑。它可應(yīng)用于多種細(xì)胞系的各種規(guī)模的轉(zhuǎn)染。與市場(chǎng)上其他的轉(zhuǎn)染試劑相比,ATG fect solution 能夠高效率轉(zhuǎn)染多種細(xì)胞系,包括貼壁細(xì)胞和懸浮細(xì)胞,尤其對(duì)于懸浮細(xì)胞(如 293-F、CHO-S 等)表現(xiàn)出卓越的性能,同時(shí)也具有具有較低的細(xì)胞毒性。 ATGfect solution 是用于瞬時(shí)和穩(wěn)定轉(zhuǎn)染的重組蛋白生產(chǎn)的理想選擇。
儲(chǔ)存條件(Storage)
請(qǐng)于-20 °C 保存,保質(zhì)期一年。
運(yùn)輸方式(Shipping)
藍(lán)冰運(yùn)輸
Note
For research use only .
狀態(tài)(Form)
Liquid
使用方法(Standard Operating Procedure)
PROTOCOL FOR SUSPENSION CELLS:
Preparation: 18 to 24 hours before transfection, seed cells at 1.0 x 106 per mL of culture. Sample Transfection.Protocol (1000 mL culture in 3L shake flask).
1. Prepare ATG fect solution-DNA transfection mixture (order is critical):
(1) Add 500 μg of plasmid DNA into100 mL FBS-free medium.
(2) Briefly mix/vortex solution.
(3) Add 1.5 mL of ATGfect solution to mixture.
(4) Vortex solution for 5 seconds.
(5) Let solution sit for 20 minutes in hooded environment to allow ATG fect solution -DNA complexes to form.
(6) Gently mix solution by pipetting up and down 3 times.
2. Add entire transfection solution to 1000 mL of cell suspension culture.
3. Return cell suspension to incubator.
4. Recombinant protein can be analysed 4-6 days after transfection.
Note: The above operation is for reference only. Please explore the ratio of transfection reagent to DNA according to your experimental needs to find the best transfection conditions.