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  • 產(chǎn)品名稱： Anti NOTCH1(M2011R) Mouse Monoclonal Antibody
  • 產(chǎn)品貨號： CST26444
  • 貨期： 現(xiàn)貨
  • 價格與訂購： 4800
  • 數(shù)量：
    庫存： 5
  • 規(guī)格： 100μg
  • 產(chǎn)品信息
  • 如何訂購
    Catalog Number
    CST26444
    Gene Symbol
    NOTCH1, AOS5, AOVD1,TAN1, hN1
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    Description
    Anti NOTCH1(M2011R) Mouse Monoclonal Antibody
    Background
    NOTCH1 is a human gene encoding a single-pass transmembrane receptor. It is a member of the NOTCH family. NOTCH family members play a role in a variety of developmental processes by controlling cell fate decisions. Diseases associated with NOTCH1 include aortic valve disease 1 and adams-oliver syndrome 5, and so on. An important paralog of this gene is NOTCH2.
    Immunogen
    A synthetic peptide from the internal region of NOTCH1 which includes the mutation of M2011R, human origin.
    Applications
    ELISA, WB, IF, IHC
    Recommended Dilutions
    ELISA: 1:1000-1:2000 
    WB: 1:500-1:1000 
    IF: 1:50-1:100 
    IHC: 1:50-1:100
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity
    Recognizes M2011R mutant, but not wild-type NOTCH1 of vertebrates.
    Immunoprecipitation/Western Blot

    Western blot analysis of recombinant NOTCH1(M2011R) and wildtype proteins. Purified His-tagged NOTCH1(M2011R) protein (lane1) and corresponding wild-type protein (lane2) were blotted with Anti NOTCH1(M2011R) monoclonal antibody (Cat. #26444).                                                 
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    Immunofluorescence: 

    Immunofluorescence of cells expressing NOTCH1 proteins with Anti NOTCH1(M2011R) antibody. HEK293T cells were transfected with pCDNA3-GFP NOTCH1(WT) plasmid (left column) or pCDNA3-GFP NOTCH1(M2011R) plasmid (right column), then fixed and stained with Anti NOTCH1(M2011R) monoclonal antibody (Cat. #26444).
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.