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  • 產(chǎn)品名稱： Anti PIK3CA(E545G) Mouse Monoclonal Antibody
  • 產(chǎn)品貨號(hào)： CST26208
  • 貨期： 現(xiàn)貨
  • 價(jià)格與訂購(gòu)： 4800
  • 數(shù)量：
    庫(kù)存： 5
  • 規(guī)格： 100μg
  • 產(chǎn)品信息
  • 如何訂購(gòu)
    Catalog Number
    CST26208
    Gene Symbol
    PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
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    Description
     Anti PIK3CA(E545G) Mouse Monoclonal Antibody
    Background
    Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
    Immunogen
    A synthetic peptide from the internal region of PIK3CA which includes the mutation of E545G, human origin.
    Applications
    ELISA, WB, IHC
    Recommended Dilutions
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity
    Recognizes E545G mutant, but not wild-type PIK3CA of vertebrates.
    Immunoprecipitation/Western Blot

    Western blot analysis of recombinant PIK3CA(E545G) and wildtype proteins. Purified His-tagged PIK3CA(E545G) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti PIK3CA(E545G) monoclonal antibody (Cat. #26208).        
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    Immunofluorescence: 

    Immunofluorescence of cells expressing PIK3CA proteins with Anti PIK3CA(E545G) antibody. HEK293T cells were transfected with pCDNA3-GFP PIK3CA(WT) plasmid (left column) or pCDNA3-GFP PIK3CA(E545G) plasmid (right column), then fixed and stained with Anti PIK3CA(E545G) monoclonal antibody (Cat. #26208).
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    Storage Conditions
    Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.